Zebrafish sperm cryopreservation with N,N-dimethylacetamide
نویسندگان
چکیده
منابع مشابه
Zebrafish sperm cryopreservation with N,N-dimethylacetamide.
High fecundity, rapid generation time, and external development of optically clear embryos make the zebrafish (Danio rerio) a convenient vertebrate model for genetic, developmental, and disease studies. Efficient sperm cryopreservation enhances the zebrafish model system by optimizing productive use of facility space, extending the reproductive lifetime of males, providing an alternative to liv...
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The laboratory rat, Rattus norvegicus, was the first mammalian species domesticated for scientific research, which work dating back to before 1850. From this auspicious beginning, the rat has become the most widely studied experimental animal model for biomedical research (Jacob, 1999). Since the development of the first inbred rat strain by King 1909, over 500 inbred rat strains have been deve...
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As the demand for assisted reproductive techniques in humans increases, so does the demand for the oocytes and sperm that are essential for these techniques. Human sperm banks play a key role in assisted human reproduction, as a reservoir of sperm from the semen of donors and as an option for genetic preservation for some patients. There are different techniques that can be used to store human ...
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OBJECTIVES To review a large experience with sperm cryopreservation in patients with testicular cancer and determine the effect of clinical stage and tumor histologic features on semen quality. METHODS The prefreeze and post-thaw sperm quality of 157 patients with testicular cancer was compared with that of 50 normal donors. The impact of tumor stage and histologic features (pure seminoma, pu...
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Sperm cryopreservation is a great challenge, since many sperm are irreversibly damaged or present altered functionality after the whole process. Although components of extenders for sperm cryopreservation are quite similar between species, sperm from each of the species present peculiarities that force researchers to optimize the extenders and protocols for each particular species. In this revi...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2003
ISSN: 0736-6205,1940-9818
DOI: 10.2144/03355st03